大鼠内侧视前区LHRH神经元儿茶酚胺能神经支配的电镜细胞化学。

T Watanabe, Y Nakai
{"title":"大鼠内侧视前区LHRH神经元儿茶酚胺能神经支配的电镜细胞化学。","authors":"T Watanabe,&nbsp;Y Nakai","doi":"10.1679/aohc.50.103","DOIUrl":null,"url":null,"abstract":"<p><p>The synaptic interactions between catecholaminergic terminals and luteinizing hormone releasing hormone (LHRH)-containing neurons in the medial preoptic area of the rat was studied by electron microscopy using LHRH immunocytochemistry combined with 5-hydroxydopamine labeling or autoradiography after injection of 3H-dopamine or 3H-noradrenaline in the same tissue section. Axon terminals labeled with 5-hydroxydopamine, 3H-dopamine or 3H-noradrenaline were found to make synapse-like contacts with LHRH-immunoreactive nerve cell bodies and fibers in the medial preoptic area and also 5-hydroxydopamine-labeled terminals made synaptic contacts with the same LHRH-immunoreactive nerve fibers with unlabeled terminals. These findings suggest that catecholaminergic neurons may innervate LHRH-containing neurons to regulate LHRH secretion via synapses with other unknown neurons in the medial preoptic area of the rat.</p>","PeriodicalId":8387,"journal":{"name":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","volume":"50 1","pages":"103-12"},"PeriodicalIF":0.0000,"publicationDate":"1987-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.50.103","citationCount":"31","resultStr":"{\"title\":\"Electron microscopic cytochemistry of catecholaminergic innervation of LHRH neurons in the medial preoptic area of the rat.\",\"authors\":\"T Watanabe,&nbsp;Y Nakai\",\"doi\":\"10.1679/aohc.50.103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The synaptic interactions between catecholaminergic terminals and luteinizing hormone releasing hormone (LHRH)-containing neurons in the medial preoptic area of the rat was studied by electron microscopy using LHRH immunocytochemistry combined with 5-hydroxydopamine labeling or autoradiography after injection of 3H-dopamine or 3H-noradrenaline in the same tissue section. Axon terminals labeled with 5-hydroxydopamine, 3H-dopamine or 3H-noradrenaline were found to make synapse-like contacts with LHRH-immunoreactive nerve cell bodies and fibers in the medial preoptic area and also 5-hydroxydopamine-labeled terminals made synaptic contacts with the same LHRH-immunoreactive nerve fibers with unlabeled terminals. These findings suggest that catecholaminergic neurons may innervate LHRH-containing neurons to regulate LHRH secretion via synapses with other unknown neurons in the medial preoptic area of the rat.</p>\",\"PeriodicalId\":8387,\"journal\":{\"name\":\"Archivum histologicum Japonicum = Nihon soshikigaku kiroku\",\"volume\":\"50 1\",\"pages\":\"103-12\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1679/aohc.50.103\",\"citationCount\":\"31\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archivum histologicum Japonicum = Nihon soshikigaku kiroku\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1679/aohc.50.103\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archivum histologicum Japonicum = Nihon soshikigaku kiroku","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1679/aohc.50.103","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 31

摘要

在同一组织切片注射3h -多巴胺或3h -去甲肾上腺素后,采用LHRH免疫细胞化学联合5-羟多巴胺标记或放射自显影技术,电镜观察大鼠内侧视前区儿茶酚胺能末端与含黄体生成素释放激素(LHRH)神经元之间的突触相互作用。发现5-羟多巴胺、3h -多巴胺或3h -去甲肾上腺素标记的轴突末端与lhrh免疫反应神经细胞体和内侧视前区纤维形成突触样接触,5-羟多巴胺标记的末端与未标记的末端与相同的lhrh免疫反应神经纤维形成突触接触。这些发现表明,儿茶酚胺能神经元可能通过与大鼠内侧视前区其他未知神经元的突触,支配含有LHRH的神经元来调节LHRH的分泌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Electron microscopic cytochemistry of catecholaminergic innervation of LHRH neurons in the medial preoptic area of the rat.

The synaptic interactions between catecholaminergic terminals and luteinizing hormone releasing hormone (LHRH)-containing neurons in the medial preoptic area of the rat was studied by electron microscopy using LHRH immunocytochemistry combined with 5-hydroxydopamine labeling or autoradiography after injection of 3H-dopamine or 3H-noradrenaline in the same tissue section. Axon terminals labeled with 5-hydroxydopamine, 3H-dopamine or 3H-noradrenaline were found to make synapse-like contacts with LHRH-immunoreactive nerve cell bodies and fibers in the medial preoptic area and also 5-hydroxydopamine-labeled terminals made synaptic contacts with the same LHRH-immunoreactive nerve fibers with unlabeled terminals. These findings suggest that catecholaminergic neurons may innervate LHRH-containing neurons to regulate LHRH secretion via synapses with other unknown neurons in the medial preoptic area of the rat.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Lysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens. Histochemical visualization of lymphatic capillaries in the rat: a comparison of methods demonstrated at the posterior pharyngeal surface. Desmin distribution in the cardiac outflow tract of the chick embryo during aortico-pulmonary septation. Three-dimensional organization of the connective tissue fibers of the human pancreas: a scanning electron microscopic study of NaOH treated-tissues. Blood vascular architecture of the rat parathyroid glands: a scanning electron microscopic study of corrosion casts.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1