研究胚胎细胞动力学的免疫细胞化学方法。

Basic and applied histochemistry Pub Date : 1988-01-01

A Casasco, A Calligaro, E Casasco, C Marchetti, P Poggi, M Casasco

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引用次数: 0

摘要

通过将溴脱氧尿苷单克隆抗体纳入s期细胞的免疫细胞化学检测，可以识别增殖细胞。在这项研究中，我们证明胚胎组织的标记是通过在1小时标记期后单次给母BrdUrd来实现的。这种简单快速的技术有利于胚胎内增殖细胞的检测，用于发育组织和胚胎细胞动力学的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An immunocytochemical method for studying embryo cytokinetics.

The immunocytochemical detection by a monoclonal antibody of bromodeoxyuridine incorporated into S-phase cells allows the identification of proliferating cells. In this study we demonstrate that the labelling of embryo tissues is achieved by a single administration of BrdUrd to the mother after a 1 hour-labelling period. This simple and rapid technique facilitates the detection of proliferating cells within the embryo for the study of developing tissues and embryo cytokinetics.

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Basic and applied histochemistry

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