单克隆抗TXB2抗体:在固、液相放射检测中的应用

Prostaglandins, leukotrienes, and medicine Pub Date : 1987-12-01 DOI:10.1016/0262-1746(87)90140-5

Elizabeth R. Hall, Caryl L. Lane, D. Scott Linthicum

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引用次数: 4

摘要

已制备并鉴定了血栓素B2 (TXB2)单克隆抗体。使用这些单克隆抗体中的一种，已经开发了液体和固相放射免疫测定。两种方法在测定11份血清样本的TXB2时结果相似;然而，在B/B0 = 90%时，固相法比液相法更敏感(即63 pg/ml vs 19 pg/ml)。尽管两种检测系统的敏感性不同，但PGD2、PGE2、PGF2α和6酮- pgf1 α单克隆抗体的交叉反应性是相同的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Monoclonal antibody against TXB2: Its use in solid and liquid phase radirmmunoassays

Monoclonal antibodies to thromboxane B₂ (TXB₂) have been produced and characterized. Both liquid and solid phase radio immunoassays have been developed using one of these monoclonal antibodies. The two assays gave similar results when used to quantitate TXB₂ in 11 serum samples; however the solid phase assay was more sensitive than the liquid phase assay (i.e., 63 pg/ml vs 19 pg/ml) at a B/B₀ = 90%). Despite a difference in the sensitivity of the two assay systems, the cross-reactivity of the monoclonal antibody for PGD₂, PGE₂, PGF_2α and 6 keto-PGF_1α was the same.

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Prostaglandins, leukotrienes, and medicine

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