大鼠胰腺腺泡细胞抗体的制备与鉴定。

P Burger, G Letko, H U Schulz, W A Müller, P Heinrich
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引用次数: 0

摘要

在大鼠急性胰腺炎过程中,免疫因子对细胞的损伤和自身消化的诱导应予以重视。从大鼠胰腺中分离完整的腺泡细胞,为制备针对细胞膜外抗原的特异性更强的抗血清提供了新的途径。采用间接免疫荧光反应法(IFAR)检测循环抗体。在体外观察到腺泡细胞表面有明确的膜荧光,在体内观察到腺泡小叶间隔有荧光,标志着腺泡的边界。
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Production and characterization of antibodies to rat pancreatic acinar cells.

The contribution of immunologic noxae to the damage of the cells and the induction of autodigestion in the course of acute pancreatitis in rats should be regarded. The isolation of intact acinar cells from rat pancreas offers a new approach to a more specific antiserum being directed against antigens at the outside of the cell membrane. Proof of circulating antibodies was performed using the indirect immunofluorescence reaction method (IFAR). A defined membrane fluorescence was observed at the surface of the acinar cells in vitro and a fluorescence at the septa of lobules marking the borders of acini in vivo.

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