淀粉或改性血红蛋白重度血液稀释后大鼠骨髓超微结构。

Scanning electron microscopy Pub Date : 1986-01-01

F R Campbell, R D Rink

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引用次数: 0

摘要

以淀粉或改性血红蛋白溶液交换输注至红细胞比容10 ~ 15时，观察大鼠骨髓超微结构。输血大鼠的血涂片有17-20%的网织红细胞，而假手术对照组为5-6%。在输注的大鼠骨髓巨噬细胞中有大量的异溶酶体，显然含有输注溶液中的淀粉或血红蛋白。内皮细胞和网状细胞也有一些被认为含有淀粉或血红蛋白的异溶酶体。经输血的大鼠网状细胞中含有大量的糖原颗粒，散布在细胞质中或成块状排列。糖原的合成可能表明网状细胞对交换输血过程引起的组织缺氧的代谢变化。

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Ultrastructure of bone marrow of rats after severe hemodilution with starch or modified hemoglobin.

The ultrastructure of bone marrow of rats was studied 24 h after exchange-transfusion with solutions of starch or modified hemoglobin to a hematocrit of 10-15. Blood smears of the transfused rats had 17-20% reticulocytes as compared to 5-6% for sham operated controls. In the transfused rats marrow macrophages had numerous heterolysosomes apparently containing the starch or hemoglobin from the transfused solutions. Endothelial cells and reticular cells also possessed a few heterolysosomes thought to contain starch or hemoglobin. Reticular cells of the transfused rats contained numerous glycogen particles scattered throughout the cytoplasm or arranged in large masses. Synthesis of glycogen may indicate a metabolic change in reticular cells in response to tissue hypoxia induced by the exchange-transfusion procedure.

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Scanning electron microscopy

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