动物胚胎系统热休克基因表达。

J J Heikkila, L W Browder, L Gedamu, R W Nickells, G A Schultz
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引用次数: 71

摘要

我们研究了热休克或应激基因在鱼类、棘皮动物、两栖动物和哺乳动物胚胎系统中的表达。在奇努克鲑鱼胚胎细胞系中,提高孵育温度或暴露于金属离子(如镉和锌)可诱导一组热休克蛋白HSPs。转录抑制剂、体外翻译和Northern杂交研究表明,鱼类热sp的合成受转录水平的调控。在斑草、非洲爪蟾、小鼠和家兔的发育早期,热休克蛋白的合成是一个阶段依赖性的现象。在每一个发育系统中,热休克蛋白的合成直到卵裂阶段之后才能被诱导。胚胎经受热休克反应(即热休克蛋白合成)的能力与通过体外翻译或Northern杂交检测热休克蛋白mRNA积累的能力相关。因此,热休克蛋白的阶段依赖性合成似乎在转录水平上受到控制。最后,在所研究的所有生物体中,合成HSP和积累HSP mRNA的能力也与耐热性的获得一致。
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Heat-shock gene expression in animal embryonic systems.

We have examined the expression of heat shock or stress genes in fish, echinoderm, amphibian, and mammalian embryonic systems. In a Chinook salmon embryo cell line, elevation of the incubation temperature or exposure to metal ions (e.g., cadmium and zinc) induced a set of heat-shock proteins HSPs. Transcriptional inhibitor, in vitro translation, and Northern hybridization studies suggest that fish HSP synthesis is regulated at the transcriptional level. The synthesis of HSPs during early development of Arbacia punctulata, Xenopus laevis, mouse, and rabbit is a stage-dependent phenomenon. In each of the developmental systems, HSP synthesis could not be induced until after cleavage stages. The ability of the embryo to undergo a heat-shock response (i.e., HSP synthesis) was correlated with the ability to detect HSP mRNA accumulation by either in vitro translation or Northern hybridization assays. Thus, the stage-dependent synthesis of HSPs appears to be controlled at the transcriptional level. Finally, in all of the organisms studied, the capacity to synthesize HSPs and accumulate HSP mRNA also coincides with acquisition of thermotolerance.

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