Early functions of poliovirus. I. The inhibition of cellular protein synthesis in HeLa cells after infection with active and inactivated poliovirus.

Evidence is presented that the genome of the infecting poliovirus must be intact in order to achieve inhibition of cellular protein synthesis. Viral particles with a single lethal hit by treatment with hydroxylamine no longer participate in the inhibitory action of the virus. De novo protein synthesis is required for the inhibition to occur. The synthesis of cellular m-RNA is not necessary.