牛皮质骨糖蛋白(骨唾液蛋白)的物理性质

P.A. Williams, A.R. Peacocke
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引用次数: 28

摘要

骨唾液蛋白是一种从牛皮质骨中提取的含有高比例唾液酸基团的糖蛋白,已对两种不同的制备方法进行了理化研究。测定了so20、w、扩散系数、分子量、沉降系数分布、旋光色散曲线、自由边界电泳迁移率、部分比容和折射率增量。两种制剂的分子量略有不同,但都是小分子,多分散,但离心和电泳均均匀。它们的摩擦系数高于球形分子的预期,它们的螺旋含量很少或没有。根据现有的分析和结构信息对结果进行了讨论。
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The physical properties of a glycoprotein from bovine cortical bone (bone sialoprotein)

Physicochemical studies have been made of two different preparations of bone sialoprotein, a glycoprotein from bovine cortical bone, which contains a high proportion of sialic acid groups. Determinations have been made of s020,w, diffusion coefficient, molecular weight, sedimentation coefficient distribution, optical rotatory dispersion curves, mobility during free boundary electrophoresis, partial specific volume and refractive index increment. The two preparations differed slightly in molecular weight, but both are small molecules, polydisperse but homogeneous with respect to centrifugation and electrophoresis. Their frictional coefficients are higher than that expected for globular molecules and they have little or no helical content. The results are discussed in the light of available analytical and structural information.

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Subject index Erratum Author index Hexosamine and acid glycosaminoglycans in human teeth Acid mucopolysaccharides of tadpole tail fin and back skin
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