核磁共振光谱法观察单核苷酸与核糖核酸酶的特异相互作用

Yasuo Inoue, Sadako Inoue
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引用次数: 2

摘要

1.1. 利用核磁共振(NMR)谱分析了底物(单核苷酸)与牛胰腺核糖核酸酶I(核糖核酸嘧啶核苷-2′-转移酶(环化),EC 2.7.7.16)和taka -淀淀酶T1(核糖核酸鸟嘌呤核苷-2′-转移酶(环化),EC 2.7.7.26)的特异性相互作用。采用衬底所显示的某些低场信号的半高线宽作为相互作用程度的度量。根据已知的一级结构,首次对核糖核酸酶T1的核磁共振谱进行了测量和解释。在天然核糖核酸酶I和热变性核糖核酸酶I存在的情况下,测定了2’(3’)UMP和尿苷的光谱,发现2’(3’)UMP的信号在热处理后变窄,而尿苷的光谱基本保持不变,这表明只有2’(3’)UMP与酶分子有效相互作用,而这种相互作用在酶变性后不会发生。
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Observation of specific interaction of mononucleotides with ribonucleases by nuclear magnetic resonance spectra

  • 1.

    1. Nuclear magnetic resonance (NMR) spectra were measured to demonstrate the specific interaction of substrates (mononucleotides) with bovine pancreatic ribonuclease I (ribonucleate pyrimidinenucleotido-2′-transferase (cyclizing), EC 2.7.7.16) and Taka-Diastase ribonuclease T1 (ribonucleate guaninenucleotido-2′-transferase (cyclizing), EC 2.7.7.26).

  • 2.

    2. The line widths at half height of certain low field signals exhibited by substrates were taken as a measure of the extent of the interaction.

  • 3.

    3. The NMR spectrum of ribonuclease T1 has been measured and interpreted in the light of its known primary structure for the first time.

  • 4.

    4. The spectra of 2′(3′)UMP and uridine were measured in the presence of native ribonuclease I and thermally denaturated ribonuclease I, and it was found that 2′(3′)UMP signals underwent narrowing after heat treatment whereas the spectrum of uridine remained almost unchanged, indicating that only 2′(3′)UMP interacts effectively with the enzyme molecule and that this interaction does not occur after denaturation of the enzyme.

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