飞蛾飞行肌线粒体伴随5 ' -二磷酸腺苷磷酸化的棕榈酸酯的快速氧化

Edmund Stevenson
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引用次数: 14

摘要

本文描述了一种从南方粘虫蛾(Prodenia eridania)胸肌中分离线粒体的方法。这些线粒体能够非常迅速地氧化棕榈酸酯,并伴随腺苷5 ' -二磷酸的磷酸化。对5′-二磷酸腺苷和无机磷酸盐有绝对需要量,但添加烟酰胺-腺嘌呤二核苷酸、烟酰胺-腺嘌呤二核苷酸磷酸和肉碱均无影响。当存在高能磷酸盐陷阱和一些蛋白质时,发现最大的摄氧率。
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Rapid oxidation of palmitate with concomitant phosphorylation of adenosine 5′-diphosphate by moth flight-muscle mitochondria

A procedure is described for isolating mitochondria from the thoracic muscle of the southern armyworm moth, Prodenia eridania. These mitochondria are capable of very rapidly oxidizing palmitate with concomitant phosphorylation of adenosine 5′-diphosphate. There is an absolute requirement for adenosine 5′-diphosphate and inorganic phosphate, but added nicotinamide-adenine dinucleotide, nicotinamide-adenine dinucleotide phosphate and carnitine have no effect. Maximal rates of oxygen uptake are found when a high-energy phosphate trap and some protein are present.

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