{"title":"成人前列腺上皮细胞携带霍乱毒素的连续繁殖。","authors":"D M Peehl, T A Stamey","doi":"10.1007/BF02619671","DOIUrl":null,"url":null,"abstract":"<p><p>Reproducible subculture of adult human prostatic epithelial cells from normal, benign hyperplastic and malignant tissue has been achieved. Cholera toxin is the key component in the culture system, but use of an optimal basal medium (PFMR-4) supplemented with a high level of serum in collagen-coated dishes also improves growth and serial propagation.</p>","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 12","pages":"981-6"},"PeriodicalIF":0.0000,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02619671","citationCount":"7","resultStr":"{\"title\":\"Serial propagation of adult human prostatic epithelial cells with cholera toxin.\",\"authors\":\"D M Peehl, T A Stamey\",\"doi\":\"10.1007/BF02619671\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Reproducible subculture of adult human prostatic epithelial cells from normal, benign hyperplastic and malignant tissue has been achieved. Cholera toxin is the key component in the culture system, but use of an optimal basal medium (PFMR-4) supplemented with a high level of serum in collagen-coated dishes also improves growth and serial propagation.</p>\",\"PeriodicalId\":13317,\"journal\":{\"name\":\"In Vitro\",\"volume\":\"20 12\",\"pages\":\"981-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF02619671\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"In Vitro\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF02619671\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF02619671","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Serial propagation of adult human prostatic epithelial cells with cholera toxin.
Reproducible subculture of adult human prostatic epithelial cells from normal, benign hyperplastic and malignant tissue has been achieved. Cholera toxin is the key component in the culture system, but use of an optimal basal medium (PFMR-4) supplemented with a high level of serum in collagen-coated dishes also improves growth and serial propagation.
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