黏液物质的组织化学。技术因素)。

Annales d'anatomie pathologique Pub Date : 1980-01-01
C A Pagnini, L Bozzola
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引用次数: 0

摘要

关于用于区分黏液物质的组织化学方法和为获得更好结果而进行的某些技术修改的报告:1)在阿利新蓝反应后在ph为1的溶液中快速通过,以确保仅染色硫酸盐黏液物质;采用h.i.d法,先在FeCl中下切20段,持续10分钟;3)甲基化反应最佳时间为12 h。
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[Histochemistry of mucosubstances. Technical considerations].

A report concerning the histochemical methods used to differentiate mucosubstances and certain technical modifications made with the aim of obtaining better results : 1) rapid passage in a solution at pH1 after alcian blue reaction in order to ensure staining of sulfate mucosubstances only ; 20 plunging of sections first in FeCl for 10 minutes by the H.I.D. method ; 3) in the methylation reaction the optimum time is 12 hours.

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