{"title":"用磷酸制备希夫型so2染料试剂及其细胞化学性质。","authors":"M K Dutt","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A new method employing phosphoric acid in place of hydrochloric acid for the preparation of azure A-SO2 and toluidine blue O-SO2 has been described in this communication. It has been found that o-phosphoric acid in a weak concentration can be a suitable substitute for hydrochloric acid in the preparation of these dye-SO2 reagents. These dye-reagents produce excellent blue nuclei in human uterine tumour. Toluidine blue O-SO2 fortified with an amino aicd, glycine also produces excellent blue chromosomes in the squash preparations of grasshopper testes fixed in acetic acid-alcohol. The shelf-life of these two dye-reagents has also been presented herein.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 2","pages":"143-6"},"PeriodicalIF":0.0000,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of Schiff-type dye-SO2 reagents with phosphoric acid and their cytochemical properties.\",\"authors\":\"M K Dutt\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A new method employing phosphoric acid in place of hydrochloric acid for the preparation of azure A-SO2 and toluidine blue O-SO2 has been described in this communication. It has been found that o-phosphoric acid in a weak concentration can be a suitable substitute for hydrochloric acid in the preparation of these dye-SO2 reagents. These dye-reagents produce excellent blue nuclei in human uterine tumour. Toluidine blue O-SO2 fortified with an amino aicd, glycine also produces excellent blue chromosomes in the squash preparations of grasshopper testes fixed in acetic acid-alcohol. The shelf-life of these two dye-reagents has also been presented herein.</p>\",\"PeriodicalId\":76158,\"journal\":{\"name\":\"Microscopica acta\",\"volume\":\"84 2\",\"pages\":\"143-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microscopica acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of Schiff-type dye-SO2 reagents with phosphoric acid and their cytochemical properties.
A new method employing phosphoric acid in place of hydrochloric acid for the preparation of azure A-SO2 and toluidine blue O-SO2 has been described in this communication. It has been found that o-phosphoric acid in a weak concentration can be a suitable substitute for hydrochloric acid in the preparation of these dye-SO2 reagents. These dye-reagents produce excellent blue nuclei in human uterine tumour. Toluidine blue O-SO2 fortified with an amino aicd, glycine also produces excellent blue chromosomes in the squash preparations of grasshopper testes fixed in acetic acid-alcohol. The shelf-life of these two dye-reagents has also been presented herein.
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