Ben A. Oostra, Annika C. Arnberg, Geert Ab, Max Gruber
{"title":"转录截断DNA片段的大肠杆菌RNA聚合酶的末端链转换","authors":"Ben A. Oostra, Annika C. Arnberg, Geert Ab, Max Gruber","doi":"10.1016/0005-2787(81)90056-3","DOIUrl":null,"url":null,"abstract":"<div><p>When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of <em>Escherichia coli</em>, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.</p></div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"655 3","pages":"Pages 446-448"},"PeriodicalIF":0.0000,"publicationDate":"1981-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90056-3","citationCount":"6","resultStr":"{\"title\":\"Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment\",\"authors\":\"Ben A. Oostra, Annika C. Arnberg, Geert Ab, Max Gruber\",\"doi\":\"10.1016/0005-2787(81)90056-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of <em>Escherichia coli</em>, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.</p></div>\",\"PeriodicalId\":100164,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"volume\":\"655 3\",\"pages\":\"Pages 446-448\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-10-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2787(81)90056-3\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005278781900563\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005278781900563","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment
When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of Escherichia coli, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.