J F Cloix, E D Miller, M G Pernollet, M A Devynck, P Meyer
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[Purification of an endogenous inhibitor of sodium-potassium-ATPase].
From human plasma of healthy subject, an inhibitor of Na+, K+-ATPase was prepared, using a gel filtration followed by anion exchange chromatography and by HPLC on reverse phase. This low molecular weight (less than 1,500 dalton) inhibitor is a substance which possesses anionic charges, and is absorbed on reverse phase. It inhibits Na+, K+-ATPase activity and the 3H-ouabain binding on human red blood cells.
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