小鼠细胞Harvey ras基因在7号染色体上的分配。

D D Pravtcheva, F H Ruddle, R W Ellis, E M Scolnick
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引用次数: 13

摘要

采用Southern(2)的方法,对含有多种小鼠染色体组合的小鼠-中国仓鼠体细胞杂交体进行酶切和32p标记的Ha-ras特异性探针杂交,分析其是否存在小鼠c-Ha-ras(1)序列。杂交体中含有小鼠c-Ha-ras片段的存在与小鼠7号染色体的存在相关。
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Assignment of murine cellular Harvey ras gene to chromosome 7.

Mouse-Chinese hamster somatic cell hybrids containing various combinations of mouse chromosomes were analyzed for the presence of the mouse c-Ha-ras (1) sequences after restriction endonuclease digestion and hybridization with a 32P-labeled Ha-ras specific probe according to the procedure of Southern (2). The presence of the mouse c-Ha-ras containing fragment was correlated with the presence of mouse chromosome 7 in the hybrids.

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Coreactivation of four inactive X genes in a hamster x human hybrid and persistence of late replication of reactivated X chromosome. Transformation of temperature-sensitive growth mutant of BHK21 cell line to wild-type phenotype with hamster and mouse DNA. Assignment of murine cellular Harvey ras gene to chromosome 7. Mammalian mitochondrial mutants selected for resistance to the cytochrome b inhibitors HQNO or myxothiazol. Assignment of gene(s) coding for antigen defined by monoclonal antibody 2B2.
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