{"title":"杂交瘤上清液中免疫球蛋白的快速测定。","authors":"D Male, G Pryce","doi":"10.3109/08820138309051963","DOIUrl":null,"url":null,"abstract":"<p><p>A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309051963","citationCount":"1","resultStr":"{\"title\":\"A rapid assay for immunoglobulin in hybridoma supernatants.\",\"authors\":\"D Male, G Pryce\",\"doi\":\"10.3109/08820138309051963\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.</p>\",\"PeriodicalId\":13417,\"journal\":{\"name\":\"Immunological communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/08820138309051963\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunological communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/08820138309051963\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309051963","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A rapid assay for immunoglobulin in hybridoma supernatants.
A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.
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