肝细胞增生:中期阻滞技术应用的适用性。

J A Alabi, M R Alison
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引用次数: 0

摘要

本研究探讨了秋水仙碱中期阻滞法应用于大鼠肝脏再生生长和苯巴比妥诱导生长两种模型的可能性。当剂量为0.5 mg/kg体重(BW)时,在苯巴比妥治疗开始后3天给药时,秋水仙碱引起有丝分裂的线性积累长达90分钟;然而,这些有丝分裂包括许多后期和末期。在部分肝切除术后的不同时间给予这种剂量的秋水仙碱,没有观察到后期逃逸,尽管停止的有丝分裂总是更加碎片化,有些甚至可能早在注射后90分钟就退化到无法辨认。由此可见,稳定剂的最佳剂量与肝脏相对重量有很大关系,因此在代偿性增生过程中稳定剂的最佳剂量会持续变化。
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Liver cell hyperplasia: on the suitability of using the metaphase-arrest technique.

This study has explored the possibility of applying the metaphase-arrest method with colchicine to two models of induced liver growth in the rat, regenerative growth and phenobarbital-induced growth. At a dosage of 0.5 mg/kg body weight (BW), colchicine caused a linear accumulation of mitoses for up to 90 min when administered at 3 days after the start of phenobarbital treatment; however these mitoses included a number of anaphases and telophases. No anaphase escape was seen when this dose of colchicine was given at various times after partial hepatectomy, though the arrested mitoses were invariably more fragmented and some may have even degenerated beyond recognition as early as 90 min after injection. It is concluded that the optimal dose of stathmokinetic agent is heavily dependent on the relative liver weight, and thus would change continuously during compensatory hyperplasia.

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Abstracts of the joint meeting of the Cell Kinetics Society and the International Cell Cycle Society. 28-31 March 1990, St Louis, Missouri, U.S.A. Abstracts of the 16th meeting of the European Study Group for Cell Proliferation. 3-6 May 1989, Milan. Proceedings of the Cell Kinetics Society, thirteenth annual meeting. 29 March-1 April 1989, White Plains, New York, U.S.A. Bone marrow fibroblast colony-forming cells are osteogenic stem cells. Epidermal tissue homeostasis. III. Effect of hydrocortisone on cell pool size, cell birth rate and cell loss in normal toads and in toads deprived of the pars distalis of the pituitary gland.
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