{"title":"[百日咳毒素刺激脑腺苷酸环化酶，诱导40,000道尔顿膜蛋白的ADP核糖基化]。","authors":"G Berthillier, F Megret, J E Alouf, A Monneron","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The adenylate cyclase activity of Rat brain synaptosomal membranes is strongly activated by forskolin. This study demonstrates: 1. That GTP or its analogs, when added to forskolin, decrease the enzyme stimulation by a factor of 40 to 60%; 2. That Pertussis toxin (TP), in the presence of NAD, ADP-ribosylates a single membrane protein of 40,000 dalton apparent molecular weight in a strictly concomitant manner. This Lubrol-soluble protein has a sedimentation coefficient of 3.6 and a Stokes radius of 6 nm. An immune serum against TP blocks the NAD-glycohydrolase activity of the toxin.</p>","PeriodicalId":10622,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","volume":"297 12","pages":"575-8"},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Pertussis toxin stimulates brain adenyl cyclase and induces ADP ribosylation of a 40,000 dalton membrane protein].\",\"authors\":\"G Berthillier, F Megret, J E Alouf, A Monneron\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The adenylate cyclase activity of Rat brain synaptosomal membranes is strongly activated by forskolin. This study demonstrates: 1. That GTP or its analogs, when added to forskolin, decrease the enzyme stimulation by a factor of 40 to 60%; 2. That Pertussis toxin (TP), in the presence of NAD, ADP-ribosylates a single membrane protein of 40,000 dalton apparent molecular weight in a strictly concomitant manner. This Lubrol-soluble protein has a sedimentation coefficient of 3.6 and a Stokes radius of 6 nm. An immune serum against TP blocks the NAD-glycohydrolase activity of the toxin.</p>\",\"PeriodicalId\":10622,\"journal\":{\"name\":\"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie\",\"volume\":\"297 12\",\"pages\":\"575-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Pertussis toxin stimulates brain adenyl cyclase and induces ADP ribosylation of a 40,000 dalton membrane protein].
The adenylate cyclase activity of Rat brain synaptosomal membranes is strongly activated by forskolin. This study demonstrates: 1. That GTP or its analogs, when added to forskolin, decrease the enzyme stimulation by a factor of 40 to 60%; 2. That Pertussis toxin (TP), in the presence of NAD, ADP-ribosylates a single membrane protein of 40,000 dalton apparent molecular weight in a strictly concomitant manner. This Lubrol-soluble protein has a sedimentation coefficient of 3.6 and a Stokes radius of 6 nm. An immune serum against TP blocks the NAD-glycohydrolase activity of the toxin.
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