原代单层细胞培养中分化阻滞大鼠胎肺。“胎儿”pO2对前体掺入磷脂和激素反应的矛盾效应。

In Vitro Pub Date : 1984-08-01 DOI:10.1007/BF02619613
A K Tanswell, M G Joneja, F Possmayer, P Harding
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引用次数: 6

摘要

用不同胎龄的胎鼠进行肺细胞阻滞培养。与先前发表的在约142毫米汞柱pO2中培养的观察结果相反,在约30毫米汞柱pO2中培养,接近正常胎儿动脉pO2,提高了镀效率,并略微提高了生长速度。然而,他们没有显示出胆碱掺入磷脂的妊娠依赖性增加,未成熟的肺细胞培养对地塞米松或三碘甲状腺原氨酸(单独或联合使用)也没有反应,增加胆碱掺入饱和卵磷脂。胆碱和甘油掺入脂质表明,在pO2约为30 mm Hg的条件下,未成熟培养物的脂质合成速度成熟,尽管保留了未成熟的形态。电镜观察显示,在两个pO2中发育的未成熟培养物之间没有明显差异。这些差异背后的细胞机制尚不清楚,但表明氧张力可能显著影响未成熟肺脂质合成的体外研究结果。
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Differentiation-arrested rat fetal lung in primary monolayer cell culture. IV. Paradoxical effect of a "fetal" pO2 on precursor incorporation into phospholipids and hormone responsiveness.

Differentiation-arrested lung cell cultures were developed from fetal rats of various gestational ages. In contrast to previously published observations with cultures in a pO2 of approximately 142 mm Hg, cultures developed in a pO2 of approximately 30 mm Hg, close to the normal fetal arterial pO2, have improved plating efficiency and a slightly increased growth rate. They did not, however, show gestation-dependent increases of choline incorporation into phospholipids, nor did immature lung cell cultures respond to dexamethasone or triiodothyronine, singly or in combination, by increased choline incorporation into saturated lecithin. The incorporation of choline and glycerol into lipids suggested a mature rate of lipid synthesis by immature cultures at a pO2 approximately 30 mm Hg, despite preservation of an immature morphology. Electron microscope observations revealed no gross differences between immature cultures developed at either pO2. The cellular mechanisms underlying these differences are unclear but suggest that oxygen tension may significantly influence results obtained with in vitro studies of lipid synthesis by immature lung.

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