从正常人类黑素细胞培养中选择性消除成纤维细胞。

In Vitro Pub Date : 1984-05-01 DOI:10.1007/BF02619590

R Halaban, F D Alfano

{"title":"从正常人类黑素细胞培养中选择性消除成纤维细胞。","authors":"R Halaban, F D Alfano","doi":"10.1007/BF02619590","DOIUrl":null,"url":null,"abstract":"The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.","PeriodicalId":13317,"journal":{"name":"In Vitro","volume":"20 5","pages":"447-50"},"PeriodicalIF":0.0000,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02619590","citationCount":"71","resultStr":"{\"title\":\"Selective elimination of fibroblasts from cultures of normal human melanocytes.\",\"authors\":\"R Halaban, F D Alfano\",\"doi\":\"10.1007/BF02619590\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.\",\"PeriodicalId\":13317,\"journal\":{\"name\":\"In Vitro\",\"volume\":\"20 5\",\"pages\":\"447-50\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF02619590\",\"citationCount\":\"71\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"In Vitro\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF02619590\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"In Vitro","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF02619590","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 71

摘要

在体外建立纯正常人类黑色素细胞的主要障碍是成纤维细胞对培养物的污染。这个障碍可以通过基因素(G418硫酸盐)选择性破坏成纤维细胞来克服。用该药物以100微克/毫升的浓度混合培养2天，可获得正常人类黑素细胞的纯培养。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Selective elimination of fibroblasts from cultures of normal human melanocytes.

The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

In Vitro

自引率

0.00%

发文量