{"title":"纯化K88ab和K88ad抗原制备特异性大肠杆菌K88抗血清。","authors":"P A Guinée, F R Mooi, W H Jansen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.</p>","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"248 2","pages":"182-9"},"PeriodicalIF":0.0000,"publicationDate":"1980-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.\",\"authors\":\"P A Guinée, F R Mooi, W H Jansen\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.</p>\",\"PeriodicalId\":23929,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"volume\":\"248 2\",\"pages\":\"182-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.
Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.
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