图像增强在活细胞低水平荧光研究中的应用。

Microscopica acta Pub Date : 1980-03-01
G T Reynolds
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引用次数: 0

摘要

在传统胶片记录不可行的情况下,使用图像增强技术可以实现对活细胞弱荧光的显微观察。简要描述了所使用的实验安排,包括在胶片或电视录像上交替记录增强器输出。文中对特定体系作了较为详细的描述,并给出了用氯四环素荧光法检测海曼草中Ca++的一个例子。
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Applications of image intensification to low level fluorescence studies of living cells.

Microscopic observations of weak fluorescence from living cells can be achieved by using image intensification techniques in situations where conventional film recording is not feasible. A brief description is given of experimental arrangements that have been used, involving recording the intensifier output alternately on film, or TV vidicons. References are given to more detailed descriptions of particular systems, and an example is presented of the detection of Ca++ in Haemanthus by means of the fluorescence of chlorotetracycline.

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