【补体对精子固定试验敏感性的作用(作者译)】。

T Kamata, K Ikuma, K Kubota, K Koyama, S Isojima
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引用次数: 0

摘要

对于不明原因不孕妇女血清中精子固定化抗体的检测,Isojima精子固定化试验被广泛应用,但各实验室的阳性率差异较大。为分析影响试验灵敏度的因素,对补体来源和补体量进行了研究,得到如下结果:1. 以溶血效价大于200 CH50的豚鼠血清为最佳。2. 大鼠和兔血清中CH50的含量远低于豚鼠,对该试验具有较好的敏感性。3.人血清浓度为34.9 CH50/ml,小鼠血清浓度低于5 CH50/ml,均不能用于精子固定试验。人血清(AB)浓缩至豚鼠血清CH50水平,在精子固定试验中表现不佳。4. 当补体来源相同时,CH50(溶血效价)与50%精子固定效价(SI50)呈正相关,补体来源不同时,CH50与SI50无相关性。5. 结果表明,精子固定化试验需要10 CH50以上的豚鼠血清才能获得最敏感的结果。
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[Role of complement for the sensitivity of sperm immobilization test (author's transl)].

For detecting the sperm immobilizing antibody in the sera of infertile women with unknown cause, Isojima's sperm immobilization test was widely used, but there was great difference on the percentage of positive results among several laboratories. For analysis of factors influencing the sensitivity of the test, source and amount of complement were studied, and the following results were obtained. 1. Guinea pig serum which contained more than 200 CH50 as hemolytic titer was best for this test. 2. Rat and rabbit sera which contained much lower CH50 than guinea pig indicated relatively good sensitivity for the test. 3. Human serum which contained 34.9 CH50/ml and mouse serum which contained less than 5 CH50/ml, both could not work on sperm immobilization test. The human serum (AB) which was concentrated to the level of guinea pigs serum as CH50 did not work well on sperm immobilization test as guinea pig serum. 4. When the complement from same source was used, the positive correlation between CH50 (hemolytic titer) and 50% sperm immobilization titer (SI50) was present, but if the complement source was different, there was no correlation between CH50 and SI50. 5. It was proved that more than 10 CH50 of guinea pig serum was necessary for sperm immobilization test to obtain the most sensitive results.

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