[亚硒酸盐和四硫酸盐肉汤与Leifson- and Wilson-Blair琼脂分离沙门氏菌的比较效果(作者译)]。

H E Müller
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引用次数: 0

摘要

对30种或血清型(S)的3581株沙门氏菌的分离结果进行统计分析,结果表明,亚硒酸盐和四硫酸盐肉汤作为富集培养基对13s的631株沙门氏菌的富集效果相似,其中11 S的2520株为四硫酸肉汤,6 S的430株为亚硒酸肉汤,其中包括两种最重要的致病种伤寒沙门氏菌和肖特氏沙门氏菌。因此,这一点需要无条件使用亚硒酸盐肉汤(表2)。在分离大多数沙门氏菌方面,Wilson-Blair琼脂比Leifson琼脂更有效(表2)。此外,Leifson琼脂上可疑菌落和假阳性菌落的百分比更高,这是由于比Wilson-Blair琼脂工作成本更高(表3)。
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[Comparative efficacies of selenite and tetrathionate broth and Leifson- and Wilson-Blair agar for the isolation of salmonellae (author's transl)].

The statistical interpretation of the isolation of 3,581 Salmonella strains belonging to 30 species or serovars (S) shows that the efficacy of selenite and tetrathionate broth as enrichment media was of similar magnitude for 631 strains for 13 S. The tetrathionate broth was better in the case of 2,520 strains from 11 S and the selenite broth in 430 strains from 6 S including the two pathogenetic most important species S. typhi and S. schottmuelleri. Therefore, this point needs the unconditional use of selenite broth (Table 2). Wilson-Blair agar was more efficient than Leifson agar in the isolation of the most salmonella (Table 2). Also the percentage of suspicious and false-positive colonies on Leifson agar is higher and is due to more expensive work than on Wilson-Blair agar (Table 3).

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