[肝脏低温保存过程中的糖酵解]。

G Otto, H Wolff, R Häcker
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引用次数: 0

摘要

用血浆蛋白组分、白蛋白、柯林斯C2、林格液和环滴灌注法低温保存猪肝6小时。检测糖酵解代谢产物:G6P、F6P、FDP、GAP、2PG、PEP、丙酮酸和乳酸。不同保存方法对糖酵解代谢物谱的改变未见。丙酮酸激酶反应对糖酵解有一定的限制。糖酵解随保存时间的延长而减少。肝切除术前人为增加糖原或糖酵解率似乎没有任何效果。
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[Glycolysis during hypothermic storage preservation of the liver].

Porcine livers were preserved for 6 hours by simple hypothermic storage using plasma-protein-fraction, albumin, Collins C2, Ringer solution, and Ring-trickle-perfusion. Following glycolytic metabolites were examined: G6P, F6P, FDP, GAP, 2PG, PEP, pyruvate, and lactate. An alteration of glycolytic metabolite-profile by the different preservation methods was not seen. There is a limitation of glycolysis by the pyruvate-kinase-reaction. Glycolysis decreases depending from preservation time. An artificial increasing of glycogen or rate of glycolysis before hepatectomy seems to be without any effect.

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