人三磷酸异构体酶序列的初步研究

Pau M. Yuan, John M. Talent, Robert W. Gracy
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引用次数: 14

摘要

利用同源肽定位和对回收肽的氨基酸分析,可以推断出人类三磷酸异构酶的大部分序列。只用不到5毫克的人酶就能达到这个效果。该酶与兔酶序列具有较强的同源性。本研究中使用的方法可以提供一种快速的程序,以获得仅在微克或毫克量的人类酶的结构信息。
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A tentative elucidation of the sequence of human triosephosphate isomerase by homology peptide mapping

By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.

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