{"title":"人三磷酸异构体酶序列的初步研究","authors":"Pau M. Yuan, John M. Talent, Robert W. Gracy","doi":"10.1016/0005-2795(81)90136-7","DOIUrl":null,"url":null,"abstract":"<div><p>By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.</p></div>","PeriodicalId":100165,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Protein Structure","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1981-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2795(81)90136-7","citationCount":"14","resultStr":"{\"title\":\"A tentative elucidation of the sequence of human triosephosphate isomerase by homology peptide mapping\",\"authors\":\"Pau M. Yuan, John M. Talent, Robert W. Gracy\",\"doi\":\"10.1016/0005-2795(81)90136-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.</p></div>\",\"PeriodicalId\":100165,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Protein Structure\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-12-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2795(81)90136-7\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Protein Structure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005279581901367\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Protein Structure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005279581901367","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A tentative elucidation of the sequence of human triosephosphate isomerase by homology peptide mapping
By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.