印度眼镜蛇毒液中毒素D的氨基酸序列

Mitsuhiro Ohta , Toyosaku Sasaki , Kyozo Hayashi
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引用次数: 8

摘要

用Sephadex G-50凝胶过滤,再用cm -纤维素层析法,从大白鲨毒液中分离出一种神经毒素D。毒素D的氨基酸序列是通过分析其还原和s -羧甲基衍生物的胰蛋白酶肽和胰蛋白酶肽来确定的。毒液含有至少四种长神经毒素(毒素A、B、C和D)和两种短神经毒素(毒素I和II),按重量计约占冻干原始毒液的16%。毒素D由单个肽链中的71个氨基酸残基组成,通过5个分子内二硫桥交联。毒素D的致死毒性约为毒素A、B、C的一半。氨基酸序列分析表明,毒素D与毒素A的不同之处在于,毒素A的-Ser-31、-Lys-35和-Arg-49残基由-Arg-31、-Lys-35和-Arg-49取代。
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The amino acid sequence of toxin D isolated from the venom of indian cobra (Naja naja)

A neurotoxin, designated toxin D, was isolated from the venom of Naja naja by gel-filtration on Sephadex G-50 followed by CM-cellulose chromatography. The amino acid sequence of toxin D was determined by analyzing tryptic and chymotryptic peptides of the reduced and S-carboxymethyl derivative. The venom contains at least four long (toxins A, B, C and D) and two short neurotoxins (toxins I and II) that account for about 16% of the lyophilized crude venom by weight. Toxin D consists of 71 amino acid residues in a single peptide chain cross-linked by five intramolecular disulfide bridges. The lethal toxicity of toxin D is found to be about half those of toxins A, B and C in the venom. Analysis of the amino acid sequence reveals that toxin D differs from toxin A in having -Arg-31, -Glu-35 and -Lys-49 in place of -Ser-31, -Lys-35 and -Arg-49 residues.

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