【材料的制备及分析用人类基因组DNA的分离】。

V Ganev, P Georgiev, L Sirakov
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引用次数: 0

摘要

本文综述了提取DNA的常用和最方便的人类样本(血细胞、成纤维细胞等)、储存条件、DNA提取方法以及评估提取DNA的质量和适用性的标准。这项工作的目的是使来自不同实验室学科的专业人员了解DNA采样和存储的基本方法,以促进他们进一步参与基于DNA技术的诊断和研究。
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[The preparation of materials and the isolation of human genomic DNA for analysis].

The work reviews the usual and most convenient human samples (blood cells, fibroblasts, etc.) for extraction of DNA, storage conditions, DNA extraction approaches, and the criteria for assessment of quality and suitability of the extracted DNA for subsequent analysis. The aim of this work is to make professionals from different laboratory disciplines aware of the basic approaches to DNA sampling and storage in order to promote their further involvement in diagnostics and research based on DNA technology.

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