荧光原位杂交技术定位人、大鼠和小鼠蛋白磷酸酶1型β催化亚基基因(PPP1CB)

Idengaku zasshi Pub Date : 1994-12-01 DOI:10.1266/jjg.69.697
M Saadat, Y Kakinoki, Y Mizuno, K Kikuchi, M C Yoshida
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引用次数: 7

摘要

采用荧光原位杂交(FISH)方法,将编码人1型蛋白磷酸酶催化亚基(PPP1CB)的基因及其对应的大鼠(PP1 delta)和小鼠(dis2m2)基因分别定位到人2p23、大鼠6q21-q23和小鼠12D上。这些结果表明PPP1CB是一个保守的共构基团的成员。结果表明,编码1型蛋白磷酸酶家族催化亚基的基因(人类的PP1 α、PP1 β和PP1 γ以及大鼠和小鼠的相应基因)尽管具有较高的同一性,但在这三个物种中位于不同的染色体上。
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Chromosomal localization of human, rat, and mouse protein phosphatase type 1 beta catalytic subunit genes (PPP1CB) by fluorescence in situ hybridization.

Using fluorescent in situ hybridization (FISH) method, gene encoding the catalytic subunit of protein phosphatase type 1 beta (PPP1CB) in human and its corresponding gene in rat (PP1 delta) and mouse (dis2m2) were mapped to human 2p23, rat 6q21-q23, and mouse 12D, respectively. These results indicate that PPP1CB is a member of conserved syntenic group. It is shown that the genes encoding catalytic subunit of protein phosphatase type 1 family (PP1 alpha, PP1 beta, and PP1 gamma in human and those corresponding genes in rat and mouse), in spite of their high identity, are located to different chromosomes in these three species.

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