猪毛滴虫和胎儿毛滴虫唾液酸结合系统的一些特性

Michal Pakandl, Libor Grubhoffer
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引用次数: 24

摘要

用猪三毛单胞菌和T. foetus对正常红细胞和酶处理红细胞的血凝作用及其抑制作用、对猪盲肠粘液的体外粘附以及神经氨酸酶的动力学特性进行了研究。所有受试菌株均能广泛粘附于猪盲肠粘液,并能凝集人(A1、A2、B和O)、兔、猪和母鸡的红细胞。不同的抑制剂在使用神经氨酸酶处理和未处理的红细胞的血凝活性(HA)试验中有效。在Scatchard图中,猪菌株41表现为独立的协同性,而牛菌株KVC-1表现为阳性的协同性。
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Some properties of sialic-acid binding systems in Tritrichomonas suis and Tritrichomonas foetus

Hemagglutination of normal and enzyme-treated red blood cells and its inhibition, in vitro adherence to porcine caecal mucus and kinetic properties of neuraminidase were carried out with Tritrichomonas suis and T. foetus. All tested strains adhered extensively to porcine caecal mucus in vitro and agglutinated human (A1, A2, B and O), rabbit, porcine and hen red blood cells. Different inhibitors were efficacious in hemagglutination activity (HA) tests using neuraminidase treated and untreated red blood cells. The Scatchard plot showed an independent type of cooperativity in porcine strain 41, while in bovine strain KVC-1, a positive type of cooperativity was observed.

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