{"title":"猪毛滴虫和胎儿毛滴虫唾液酸结合系统的一些特性","authors":"Michal Pakandl, Libor Grubhoffer","doi":"10.1016/0305-0491(94)90106-6","DOIUrl":null,"url":null,"abstract":"<div><p>Hemagglutination of normal and enzyme-treated red blood cells and its inhibition, <em>in vitro</em> adherence to porcine caecal mucus and kinetic properties of neuraminidase were carried out with <em>Tritrichomonas suis</em> and <em>T. foetus</em>. All tested strains adhered extensively to porcine caecal mucus <em>in vitro</em> and agglutinated human (A<sub>1</sub>, A<sub>2</sub>, B and O), rabbit, porcine and hen red blood cells. Different inhibitors were efficacious in hemagglutination activity (HA) tests using neuraminidase treated and untreated red blood cells. The Scatchard plot showed an independent type of cooperativity in porcine strain 41, while in bovine strain KVC-1, a positive type of cooperativity was observed.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"108 4","pages":"Pages 529-536"},"PeriodicalIF":0.0000,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90106-6","citationCount":"24","resultStr":"{\"title\":\"Some properties of sialic-acid binding systems in Tritrichomonas suis and Tritrichomonas foetus\",\"authors\":\"Michal Pakandl, Libor Grubhoffer\",\"doi\":\"10.1016/0305-0491(94)90106-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Hemagglutination of normal and enzyme-treated red blood cells and its inhibition, <em>in vitro</em> adherence to porcine caecal mucus and kinetic properties of neuraminidase were carried out with <em>Tritrichomonas suis</em> and <em>T. foetus</em>. All tested strains adhered extensively to porcine caecal mucus <em>in vitro</em> and agglutinated human (A<sub>1</sub>, A<sub>2</sub>, B and O), rabbit, porcine and hen red blood cells. Different inhibitors were efficacious in hemagglutination activity (HA) tests using neuraminidase treated and untreated red blood cells. The Scatchard plot showed an independent type of cooperativity in porcine strain 41, while in bovine strain KVC-1, a positive type of cooperativity was observed.</p></div>\",\"PeriodicalId\":100294,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"volume\":\"108 4\",\"pages\":\"Pages 529-536\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0305-0491(94)90106-6\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0305049194901066\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0305049194901066","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Some properties of sialic-acid binding systems in Tritrichomonas suis and Tritrichomonas foetus
Hemagglutination of normal and enzyme-treated red blood cells and its inhibition, in vitro adherence to porcine caecal mucus and kinetic properties of neuraminidase were carried out with Tritrichomonas suis and T. foetus. All tested strains adhered extensively to porcine caecal mucus in vitro and agglutinated human (A1, A2, B and O), rabbit, porcine and hen red blood cells. Different inhibitors were efficacious in hemagglutination activity (HA) tests using neuraminidase treated and untreated red blood cells. The Scatchard plot showed an independent type of cooperativity in porcine strain 41, while in bovine strain KVC-1, a positive type of cooperativity was observed.