经典肠致病性血清群大肠杆菌菌株的毒性和黏附特性。

Acta microbiologica Hungarica Pub Date : 1993-01-01
J Blanco, J E Blanco, M Blanco, E A González, J I Garabal, M P Alonso
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引用次数: 0

摘要

研究了23株典型肠致病性大肠杆菌(EPEC)血清群耐热性(LT)和耐热性(STa)肠毒素、维罗毒素(VT)、细胞毒性坏死因子CNF1和CNF2、α -溶血素(Hly)、兔皮肤坏死和通透性改变、小鼠致死活性、甘露糖抗性(MRHA)和甘露糖敏感性(MSHA)血凝、相对细胞表面疏水性和P菌毛的表达。23株EPEC菌株中,O18ac: H7 (Hly+)、O20: H26(致死)、O26: H- (Hly+)、O44: H18 (Hly+)、O55: H- (CNF2+、坏死和致死)、O119: H27 (VT+和Hly+)和O142: H6(致死)血清型7株(30%)产生毒性因子。23株EPEC菌株中7株(30%)为MRHA+, 17株(74%)为MSHA+,仅有2株具有高疏水性。两株血清型为O18ac: H7和O44: H18的菌株显示MRHA型IVa,在CFA培养基上生长时进行了固定。
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Toxic and adhesive properties of Escherichia coli strains belonging to classic enteropathogenic serogroups.

Twenty-three strains belonging to classic enteropathogenic Escherichia coli (EPEC) serogroups were investigated for the production of heat-labile (LT) and heat-stable (STa) enterotoxins, verotoxins (VT), cytotoxic necrotizing factors CNF1 and CNF2, alpha-haemolysin (Hly), necrosis and modification of permeability in rabbit skin, lethal activity to mice, mannose-resistant (MRHA) and mannose-sensitive (MSHA) haemagglutination, relative cell surface hydrophobicity and the expression of P fimbriae. Of 23 EPEC strains, 7 (30%) belonging to serotypes O18ac: H7 (Hly+), O20: H26 (lethal), O26: H- (Hly+), O44: H18 (Hly+), O55: H- (CNF2+, necrotic and lethal), O119: H27 (VT+ and Hly+) and O142: H6 (lethal) produced toxic factors. Seven (30%) of 23 EPEC strains were MRHA+, 17 (74%) were MSHA+ and only 2 possessed high hydrophobicity. Two strains belonging to serotypes O18ac: H7 and O44: H18 that showed MRHA type IVa were fimbriated when grown on CFA medium.

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