干扰素γ对巨噬细胞样细胞系P388D膜构象的影响。

H Darmani, J L Harwood, S K Jackson
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引用次数: 3

摘要

干扰素- γ (ifn - γ)特异性诱导不饱和脂肪酸[14C]亚油酸进入小鼠巨噬细胞样P388D细胞系的膜磷脂,但不改变饱和脂肪酸[14C]硬脂酸的掺入。自旋标签ESR光谱用于检测这些ifn - γ诱导的膜流动性变化的任何影响,结果显示质膜流动性显著增加。这种膜流动性的改变可能对细胞物理化学相互作用的动态特性产生重要影响,ifn - γ对巨噬细胞的一些刺激作用可能归因于其对质膜组成的影响。
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Effect of interferon-gamma on membrane conformation in the macrophage-like cell line P388D.

Interferon-gamma (IFN-gamma) specifically induced the uptake of the unsaturated fatty acid [14C]linoleic acid into membrane phospholipids of the murine macrophage-like P388D cell lineage, but did not alter the incorporation of the saturated fatty acid [14C]stearic acid. Spin label ESR spectroscopy was used to examine any effects of these IFN-gamma-induced changes on membrane fluidity and the results revealed significant increases in plasma membrane fluidity. This alteration in membrane fluidity may have important consequences in the dynamic properties of cellular physiochemical interactions and some of the stimulatory effects of IFN-gamma on macrophages might be attributed to its effects on the plasma membrane composition.

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