利用质粒谱和IS200分布鉴定病毒沙门氏菌噬菌体类型。

E Torre, E J Threlfall, M D Hampton, L R Ward, I Gibert, B Rowe
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引用次数: 24

摘要

通过质粒谱和插入序列IS200的分布对57种病毒沙门氏菌噬菌体型菌株进行了鉴定。鉴定出32株携带质粒,21种型别;17株对抗菌药物耐药。相比之下,只有6个类型菌株携带IS200基因,鉴定出3种模式。Salm之内。Virchow噬菌体31型,10个野生型分离株中有5个携带质粒,鉴定出2个质粒谱;相比之下,在这些菌株中只有一个基因组中发现了IS200元素。得出结论:对于Salm。virchow, IS200不太可能显著延长噬菌体分型所达到的区分程度,在适当的时候可以通过质粒谱分型来补充。
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Characterization of Salmonella virchow phage types by plasmid profile and IS200 distribution.

The type strains of the 57 phage types of Salmonella virchow have been characterized by plasmid profile and by distribution of the insertion sequence IS200. Thirty-two strains carried plasmids and 21 profile types were identified; 17 strains were resistant to antimicrobial agents. In contrast only six of the type strains carried IS200 elements and three patterns were identified. Within Salm. virchow phage type 31, five of 10 wild-type isolates carried plasmids and two plasmid profiles were identified; in contrast, an IS200 element was identified in the genome of only one of these strains. It is concluded that for Salm. virchow, IS200 is unlikely to significantly extend the degree of discrimination achieved by phage typing which may be supplemented when appropriate by plasmid profile typing.

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