W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay
{"title":"Renilla reniformis荧光素酶基因在哺乳动物细胞中的表达。","authors":"W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay","doi":"10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M","DOIUrl":null,"url":null,"abstract":"<p><p>A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.</p>","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":"11 1","pages":"31-7"},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"90","resultStr":"{\"title\":\"Expression of the Renilla reniformis luciferase gene in mammalian cells.\",\"authors\":\"W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay\",\"doi\":\"10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.</p>\",\"PeriodicalId\":15068,\"journal\":{\"name\":\"Journal of bioluminescence and chemiluminescence\",\"volume\":\"11 1\",\"pages\":\"31-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"90\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of bioluminescence and chemiluminescence\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of bioluminescence and chemiluminescence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1099-1271(199601)11:1<31::AID-BIO398>3.0.CO;2-M","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression of the Renilla reniformis luciferase gene in mammalian cells.
A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.