Renilla reniformis荧光素酶基因在哺乳动物细胞中的表达。

W W Lorenz, M J Cormier, D J O'Kane, D Hua, A A Escher, A A Szalay
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引用次数: 90

摘要

一个编码Renilla reniformis荧光素酶的cDNA分别在类似动物和小鼠细胞中短暂和稳定地表达。在体外和体内均检测到了荧光素酶催化的荧光发射。本工作建立了一种新的有效的哺乳动物细胞荧光素酶基因表达标记。
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Expression of the Renilla reniformis luciferase gene in mammalian cells.

A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.

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Bioluminescence: Methods and Protocols, Volume 2 Bioluminescence: Methods and Protocols, Volume 1 Luminous Fungi BACK MATTER Luminous Mollusca
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