低温蒸汽甲醛灭菌生物学指标:温度和甲醛浓度变化对嗜热脂肪芽孢杆菌NCIMB 8224孢子影响的研究

A M Wright, E V Hoxey, C J Soper, D J Davies
{"title":"低温蒸汽甲醛灭菌生物学指标:温度和甲醛浓度变化对嗜热脂肪芽孢杆菌NCIMB 8224孢子影响的研究","authors":"A M Wright,&nbsp;E V Hoxey,&nbsp;C J Soper,&nbsp;D J Davies","doi":"10.1111/j.1365-2672.1996.tb03218.x","DOIUrl":null,"url":null,"abstract":"<p><p>Five strains of Bacillus stearothermophilus have been studied to identify a spore strain to be used as a biological indicator organism for low temperature steam and formaldehyde sterilization. Three strains gave poor reproducibility of batch size and growth index and were discarded. The other two strains gave good reproducibility with a high growth index and gave rise to linear survivor curves when exposed to 5% aqueous formaldehyde. However, only NCIMB 8224 sporulates on a simpler medium and as it was the most resistant to formaldehyde, it was further studied. Tests were carried out in a modified miniclave and factors studied included temperature of the steam and formaldehyde concentration. All studies confirmed the suitability of this strain as a biological indicator organism.</p>","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 3","pages":"259-65"},"PeriodicalIF":0.0000,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03218.x","citationCount":"0","resultStr":"{\"title\":\"Biological indicators for low temperature steam and formaldehyde sterilization: investigation of the effect of change in temperature and formaldehyde concentration on spores of Bacillus stearothermophilus NCIMB 8224.\",\"authors\":\"A M Wright,&nbsp;E V Hoxey,&nbsp;C J Soper,&nbsp;D J Davies\",\"doi\":\"10.1111/j.1365-2672.1996.tb03218.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Five strains of Bacillus stearothermophilus have been studied to identify a spore strain to be used as a biological indicator organism for low temperature steam and formaldehyde sterilization. Three strains gave poor reproducibility of batch size and growth index and were discarded. The other two strains gave good reproducibility with a high growth index and gave rise to linear survivor curves when exposed to 5% aqueous formaldehyde. However, only NCIMB 8224 sporulates on a simpler medium and as it was the most resistant to formaldehyde, it was further studied. Tests were carried out in a modified miniclave and factors studied included temperature of the steam and formaldehyde concentration. All studies confirmed the suitability of this strain as a biological indicator organism.</p>\",\"PeriodicalId\":22599,\"journal\":{\"name\":\"The Journal of applied bacteriology\",\"volume\":\"80 3\",\"pages\":\"259-65\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03218.x\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of applied bacteriology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/j.1365-2672.1996.tb03218.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of applied bacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1365-2672.1996.tb03218.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

对5株嗜热脂肪芽孢杆菌进行了研究,以确定一株芽孢菌株作为低温蒸汽和甲醛灭菌的生物指示菌。3株菌种的批量和生长指数重现性较差,被丢弃。另外两株菌株在5%甲醛水溶液条件下繁殖性好,生长指数高,存活曲线呈线性。然而,只有NCIMB 8224在更简单的培养基上产生孢子,并且由于其对甲醛的抗性最强,因此对其进行了进一步的研究。试验是在一个改进的微型容器中进行的,研究的因素包括蒸汽温度和甲醛浓度。所有的研究都证实了该菌株作为生物指示生物的适宜性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Biological indicators for low temperature steam and formaldehyde sterilization: investigation of the effect of change in temperature and formaldehyde concentration on spores of Bacillus stearothermophilus NCIMB 8224.

Five strains of Bacillus stearothermophilus have been studied to identify a spore strain to be used as a biological indicator organism for low temperature steam and formaldehyde sterilization. Three strains gave poor reproducibility of batch size and growth index and were discarded. The other two strains gave good reproducibility with a high growth index and gave rise to linear survivor curves when exposed to 5% aqueous formaldehyde. However, only NCIMB 8224 sporulates on a simpler medium and as it was the most resistant to formaldehyde, it was further studied. Tests were carried out in a modified miniclave and factors studied included temperature of the steam and formaldehyde concentration. All studies confirmed the suitability of this strain as a biological indicator organism.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Yersinia enterocolitica. Isolation, partial characterization and mode of action of acidocin J1229, a bacteriocin produced by Lactobacillus acidophilus JCM 1229. Note: isolation, characterization and epidemiology of Yersinia enterocolitica from humans and animals. Application of antimicrobial-producing lactic acid bacteria to control pathogens in ready-to-use vegetables. The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1