{"title":"厌氧纤维素分解细菌的基因操作。","authors":"K L Anderson","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Transposon Tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. Transconjugants of E. cellulosolvens were resistant to both tetracycline and erythromycin. E. cellulosolvens could also serve as a donor for conjugal transfer of Tn1545 back into C. beijerinckii.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic manipulation of anaerobic cellulolytic bacteria.\",\"authors\":\"K L Anderson\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Transposon Tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. Transconjugants of E. cellulosolvens were resistant to both tetracycline and erythromycin. E. cellulosolvens could also serve as a donor for conjugal transfer of Tn1545 back into C. beijerinckii.</p>\",\"PeriodicalId\":77373,\"journal\":{\"name\":\"SAAS bulletin, biochemistry and biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"SAAS bulletin, biochemistry and biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"SAAS bulletin, biochemistry and biotechnology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genetic manipulation of anaerobic cellulolytic bacteria.
Transposon Tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. Transconjugants of E. cellulosolvens were resistant to both tetracycline and erythromycin. E. cellulosolvens could also serve as a donor for conjugal transfer of Tn1545 back into C. beijerinckii.
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