吲哚(褪黑素、血清素和色氨酸)对体外卟啉代谢的影响。

K Kalka, C Fritsch, K Bolsen, B Verwohlt, G Goerz
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引用次数: 5

摘要

我们检测了褪黑素、血清素和色氨酸对HaCaT、SKMel-23和HepG2细胞中基础和δ -氨基乙酰丙酸(ALA)诱导的卟啉含量的影响。ala预孵育的细胞和不含ala的细胞分别用含有1 mM褪黑素、血清素或色氨酸的培养基喂养。24 h后测定细胞和培养基中卟啉的含量。在3个细胞系中,1 mM ALA作用24 h后,所有细胞系的卟啉浓度均有不同程度的升高:HepG2 > SKMel-23 > HaCaT细胞。在HepG2细胞中,褪黑素、血清素和色氨酸对ala诱导的卟啉浓度均无显著影响,但这三种吲哚均可抑制SKMel-23和HaCaT细胞中的卟啉水平。吲哚可降低HaCat或SKMel-23细胞对ALA的摄取。另一种机制可能是抑制将ALA转化为卟啉的酶。
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Influence of indoles (melatonin, serotonin and tryptophan) on the porphyrin metabolism in vitro.

We examined the influence of melatonin, serotonin and tryptophan on the basal and delta-aminolevulinic acid (ALA)-induced porphyrin content in HaCaT, SKMel-23 and HepG2 cells. ALA-preincubated and ALA-free cells were fed with medium containing 1 mM melatonin, serotonin or tryptophan. After 24 h the porphyrin content in the cells and in the culture medium was measured. In the three cell lines the inbucation with 1 mM ALA over 24 h increased the porphyrin concentration in all cell lines in different degrees: HepG2 > SKMel-23 > HaCaT cells. In HepG2 cells, neither melatonin, serotonin nor tryptophan influenced ALA-induced porphyrin concentrations significantly, but all three indoles depressed the porphyrin levels in SKMel-23 and HaCaT cells. The indoles may decrease the ALA uptake in HaCat or SKMel-23 cells. Another mechanism could be the inhibition of enzymes converting ALA into porphyrins.

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