Epidemiology of genital Chlamydia trachomatis.

cline disc to the edge of confluent growth (table). In this study, we further characterised the tetM genes of these TRNG strains. Ison et al previously reported the primer pair (A: 5'-GGCGTACAAGCACAAACTCG-3' and B: 5'-TCTCTGTTCAGG -TTTACTCG-3') for detection of tetM in N gonorrhoeae. These sequences were derived from that of the Ureaplasma urealyticum tetM gene.2 More recently, the nucleotide sequences of the tetM genes of American and Dutch type plasmids have been determined and suggested that the tetM determinant found in the American type plasmid has a different origin from that in the Dutch type.3 Because the base sequence of the tetM gene from Dutch type plasmid which corresponds to the primer B is different from that of American type plasmid,3 primer B2 (5'-CCTTTGTTGAGGT1TGCTCG-3') was used instead of the primer B to detect Dutch type tetM. The cells grown