ROn-1 SINEs:一种由trna衍生的短穿插重复DNA元件,来自于nilochromis及其在东半球慈鲷鱼中的特定分布。

L J Bryden, E M Denovan-Wright, J M Wright
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引用次数: 0

摘要

从罗非鱼中克隆了一个sin样重复元件(ROn-1)。该元件的长度为345个碱基对(bp),由一个具有假定的RNA聚合酶III识别序列的转移RNA样结构域、一个与trna无关的区域和一个聚(a)尾部组成。niloticus的单倍体基因组中大约有6000个拷贝的ROn-1。Southern blot分析显示,ROn-1在许多非洲慈鲷基因组中是一个丰富的元素,但在印度慈鲷Etroplus基因组中缺失。
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ROn-1 SINEs: a tRNA-derived, short interspersed repetitive DNA element from Oreochromis niloticus and its species-specific distribution in Old World cichlid fishes.

A SINE-like repetitive element (ROn-1) has been cloned from the tilapiine cichlid fish Oreochromis niloticus. The element is 345 base pairs (bp) in length and consists of a transfer-RNA-like domain with putative RNA polymerase III recognition sequences, a tRNA-unrelated region, and a poly(A) tail. Approximately 6000 copies of ROn-1 occur in the haploid genome of O. niloticus. Southern blot analysis revealed that ROn-1 is an abundant element in the genomes of many African cichlid fishes, but absent from the genome of the Indian cichlid Etroplus.

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