{"title":"11-顺式LRAT、11-顺式RD和11-顺式REH在牛视网膜色素上皮膜中的分布","authors":"Nathan L. Mata, Andrew T.C. Tsin","doi":"10.1016/S0005-2760(98)00078-2","DOIUrl":null,"url":null,"abstract":"<div><p>Our recent finding of the co-localization of 11-<em>cis</em> retinyl esters and 11-<em>cis</em> retinyl ester hydrolase (11-<em>cis</em> REH) activity in bovine retinal pigment epithelium (RPE) plasma membrane (PM) has led us to explore the possibility that the PM may provide 11-<em>cis</em> retinal for rhodopsin regeneration. In the RPE, visual chromophore is synthesized via a membrane associated 11-<em>cis</em> retinol dehydrogenase (11-<em>cis</em> RD). Accordingly, bovine RPE membranes enriched with either endoplasmic reticulum (ER) or plasma membrane (PM) enzyme markers were prepared and assayed for visual cycle enzyme activities. Pronounced 11-<em>cis</em> RD activity was associated with both ER- and PM-enriched membrane fractions. In contrast, 11-<em>cis</em> REH activity was mostly recovered in PM-enriched fractions while LRAT activity was found only in ER-enriched membranes. The finding that both 11-<em>cis</em> retinol and 11-<em>cis</em> retinal can be produced at the PM of the bovine RPE strongly suggests that 11-<em>cis</em> retinyl esters at this subcellular locale serve as a precursor of visual chromophore for pigment regeneration.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00078-2","citationCount":"25","resultStr":"{\"title\":\"Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes\",\"authors\":\"Nathan L. Mata, Andrew T.C. Tsin\",\"doi\":\"10.1016/S0005-2760(98)00078-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Our recent finding of the co-localization of 11-<em>cis</em> retinyl esters and 11-<em>cis</em> retinyl ester hydrolase (11-<em>cis</em> REH) activity in bovine retinal pigment epithelium (RPE) plasma membrane (PM) has led us to explore the possibility that the PM may provide 11-<em>cis</em> retinal for rhodopsin regeneration. In the RPE, visual chromophore is synthesized via a membrane associated 11-<em>cis</em> retinol dehydrogenase (11-<em>cis</em> RD). Accordingly, bovine RPE membranes enriched with either endoplasmic reticulum (ER) or plasma membrane (PM) enzyme markers were prepared and assayed for visual cycle enzyme activities. Pronounced 11-<em>cis</em> RD activity was associated with both ER- and PM-enriched membrane fractions. In contrast, 11-<em>cis</em> REH activity was mostly recovered in PM-enriched fractions while LRAT activity was found only in ER-enriched membranes. The finding that both 11-<em>cis</em> retinol and 11-<em>cis</em> retinal can be produced at the PM of the bovine RPE strongly suggests that 11-<em>cis</em> retinyl esters at this subcellular locale serve as a precursor of visual chromophore for pigment regeneration.</p></div>\",\"PeriodicalId\":100162,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00078-2\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0005276098000782\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0005276098000782","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes
Our recent finding of the co-localization of 11-cis retinyl esters and 11-cis retinyl ester hydrolase (11-cis REH) activity in bovine retinal pigment epithelium (RPE) plasma membrane (PM) has led us to explore the possibility that the PM may provide 11-cis retinal for rhodopsin regeneration. In the RPE, visual chromophore is synthesized via a membrane associated 11-cis retinol dehydrogenase (11-cis RD). Accordingly, bovine RPE membranes enriched with either endoplasmic reticulum (ER) or plasma membrane (PM) enzyme markers were prepared and assayed for visual cycle enzyme activities. Pronounced 11-cis RD activity was associated with both ER- and PM-enriched membrane fractions. In contrast, 11-cis REH activity was mostly recovered in PM-enriched fractions while LRAT activity was found only in ER-enriched membranes. The finding that both 11-cis retinol and 11-cis retinal can be produced at the PM of the bovine RPE strongly suggests that 11-cis retinyl esters at this subcellular locale serve as a precursor of visual chromophore for pigment regeneration.
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