台湾北部2株白纹伊蚊对日本脑炎病毒的敏感性研究。

M H Weng, J C Lien, Y S Show
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摘要

白纹伊蚊是台湾北部的优势蚊种。实验室检测了2株白纹伊蚊对nt113乙型脑炎病毒的媒介能力。经胸内(i.t)接种三夏(SH)菌株的蚊子感染剂量50 (MI-D50)为-1.1 log WMICLD50(断奶小鼠脑内致死剂量),永禾(YH)菌株的感染剂量50为-2.0 log WMICLD50。SH株经itt接种50%蚊虫传播感染剂量(MTID50)为3.5 log, YH株未发生传播。经加糖血病毒混合物喂养,SH株的MID50为2.7 log,而YH株的感染率未达到50%。通过病毒血症小鼠血喂养,两种菌株的最高感染率均在30%左右。没有证据表明病毒通过口腔感染传播。
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[Studies on the susceptibility of two northern Taiwan strains of Aedes albopictus mosquitoes to Japanese encephalitis virus].

Aedes albopictus is a dominant mosquito species in northern Taiwan. In laboratory, the vector competence of 2 geographical strains of Aedes albopictus mosquitoes to NT 113 strain of Japanese encephalitis virus was examined. The mosquito infection dose50 (MI-D50) of Sanhsia (SH) strain by intrathoracic (i.t.) inoculation was shown to be -1.1 log WMICLD50 (weanling mice intracerebrum lethal dose), while that of Yungho (YH) strain was -2.0 log WMICLD50. The infection dose for 50% mosquitoes transmission (MTID50) by i.t. inoculation was 3.5 log with SH strain but no transmission occurred with YH strain. By feeding sweetened blood-virus mixture, the MID50 with SH strain was 2.7 log though YH strain did not attain 50% infection rate. By viremic mouse blood feeding, the highest infection rate for both strains was about 30%. No evidence of virus transmission was demonstrated by oral infection.

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