E Bertino, A Coscia, S Costa, D Farinasso, G Prandi, C Fabris, M Cavaletto, M G Giuffrida, A Conti
{"title":"母亲摄入的人乳中缺乏牛β -乳球蛋白。ELISA测定结果不可靠。","authors":"E Bertino, A Coscia, S Costa, D Farinasso, G Prandi, C Fabris, M Cavaletto, M G Giuffrida, A Conti","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the presence of bovine beta-LG in breast milk.</p><p><strong>Methods: </strong>Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing.</p><p><strong>Results: </strong>beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens.</p><p><strong>Conclusions: </strong>False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.</p>","PeriodicalId":6943,"journal":{"name":"Acta bio-medica de L'Ateneo parmense : organo della Societa di medicina e scienze naturali di Parma","volume":"68 Suppl 1 ","pages":"15-9"},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Absence in human milk of bovine beta-lactoglobulin ingested by the mother. Unreliability of ELISA measurements.\",\"authors\":\"E Bertino, A Coscia, S Costa, D Farinasso, G Prandi, C Fabris, M Cavaletto, M G Giuffrida, A Conti\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To analyze the presence of bovine beta-LG in breast milk.</p><p><strong>Methods: </strong>Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing.</p><p><strong>Results: </strong>beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens.</p><p><strong>Conclusions: </strong>False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.</p>\",\"PeriodicalId\":6943,\"journal\":{\"name\":\"Acta bio-medica de L'Ateneo parmense : organo della Societa di medicina e scienze naturali di Parma\",\"volume\":\"68 Suppl 1 \",\"pages\":\"15-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta bio-medica de L'Ateneo parmense : organo della Societa di medicina e scienze naturali di Parma\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta bio-medica de L'Ateneo parmense : organo della Societa di medicina e scienze naturali di Parma","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Absence in human milk of bovine beta-lactoglobulin ingested by the mother. Unreliability of ELISA measurements.
Objectives: To analyze the presence of bovine beta-LG in breast milk.
Methods: Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing.
Results: beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens.
Conclusions: False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.
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