乙型脑炎病毒蚊媒能力研究系统。

Indian journal of malariology Pub Date : 1998-09-01
P P Samuel, J Hiriyan, V Thenmozhi, A Balasubramanian
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引用次数: 0

摘要

本文描述了一种用乙型脑炎病毒(JEV)感染蚊子并在外部潜伏期后证明病毒传播的方法。采用单口喂养法,感染率可达90%以上。通过让受感染的蚊子探测一种合适的血清培养基,并对探测的血清进行病毒检测,证明了病毒的传播。昆虫生物测定法证实了感染和传播。
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A system for studying vector competence of mosquitoes for Japanese encephalitis virus.

A method to infect mosquitoes with Japanese encephalitis virus (JEV) and to demonstrate virus transmission after an extrinsic incubation period is described. Using per oral feeding method infection rate as high as 90% could be achieved. Demonstration of transmission of the virus was achieved by allowing the infected mosquitoes to probe a suitable serum medium and testing the probed serum for virus. Both infection and transmission were demonstrated by using insect-bioassay.

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