从脆弱拟杆菌肠毒素产生菌株中提取的LPS的抗原性。

Acta microbiologica Polonica Pub Date : 1999-01-01
F Meisel-Mikołajczyk, K Kot, W Kłos, G S Rouyan, M Mieszała, A Gamian
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引用次数: 0

摘要

对波兰产肠毒素脆弱拟杆菌(ETBF)与参考菌株的抗原性进行了比较。用苯酚-水提取物水相制备的脂多糖(LPS)进行凝集和被动血凝、SDS-PAGE分析和免疫印迹试验以及糖和脂肪酸分析。不同的ETBF抗原在血清学反应性上存在差异。在血清学试验中,非产肠毒素参考菌株IPL E-323抗原对ETBF菌株的全细胞与血清表现出弱的交叉反应性。ETBF和NTBF菌株在脂肪酸和糖组成上存在一定差异。LPS制剂可能具有共同的核心结构和可变链长的o特异性多糖。
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Antigenic properties of LPS extracted from Bacteroides fragilis enterotoxin producing strains.

Antigenic properties of enterotoxigenic Bacteroides fragilis (ETBF) strains isolated in Poland were compared with reference strains. The agglutination and passive hemagglutination, SDS-PAGE analysis and immunoblotting tests as well as analyses of sugars and fatty acids were performed with lipopolysaccharide (LPS) preparations obtained from water-phase of phenol-water extracts. Some differences in serological reactivity between ETBF antigens were observed. The antigen of the NTBF (nonenterotoxigenic) reference strain IPL E-323 expressed weak cross-reactivity with sera against whole cells of ETBF strains in serological tests. There were some differences observed between ETBF and NTBF strains in fatty acids and sugar composition. The LPS preparations probably possess a common core structure and the O-specific polysaccharides of variable chain length.

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