凝血酶通过涉及胞质磷脂酶A2和12/15脂氧合酶产物的途径引起假足脱离。

S Ross, B Essary, B A de la Houssaye, Z Pan, K Mikule, O Mubarak, K H Pfenninger
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引用次数: 0

摘要

凝血酶引起邓宁大鼠前列腺癌(MAT-Lu)细胞假足快速脱离和缩短。通过干涉反射显微镜和paxillin和talin抗体的免疫荧光分析可以看出,主要事件是粘附位点的分解。从生化角度看,凝血酶是胞质磷脂酶A2的有效激活剂,可以增加这些细胞中类二十烷酸的产生。脂氧合酶(但不含环氧合酶)抑制剂可阻断假足的作用。花生四烯酸和12(S)-羟二糖四烯酸或15(S)-羟二糖四烯酸模拟凝血酶的作用。我们得出结论,在某些癌细胞中,凝血酶是一种伪足驱避剂,通过涉及细胞质磷脂酶A2、12/15-脂氧合酶和12(S)-和/或15(S)-羟基二碳四烯酸的级联反应发挥作用。
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Thrombin causes pseudopod detachment via a pathway involving cytosolic phospholipase A2 and 12/15-lipoxygenase products.

Thrombin causes rapid pseudopod detachment and shortening in Dunning rat prostatic carcinoma (MAT-Lu) cells. As seen by interference reflection microscopy and by immunofluorescence analysis with antibodies to paxillin and talin, the primary event is disassembly of adhesion sites. Biochemically, thrombin is a potent activator of cytosolic phospholipase A2 and increases eicosanoid production in these cells. The pseudopod effects are blocked by lipoxygenase (but not cyclooxygenase) inhibitors. Arachidonic acid and 12(S)-hydroxyeicosatetraenoic acid or 15(S)-hydroxyeicosatetraenoic acid mimic the thrombin effect. We conclude that in certain cancer cells, thrombin is a pseudopod repellent that exerts its effect via a cascade involving cytosolic phospholipase A2, 12/15-lipoxygenase, and 12(S)- and/or 15(S)-hydroxyeicosatetraenoic acid.

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