Immunofluorescence and enzyme immunomicroscopy methods.

Immunohistochemistry is a very versatile immunopathological tool for the study of distribution and differentiation of antigens and of the presence of in vivo-bound immune complexes. In addition, these methods are invaluable for detection of circulating antibodies to the various antigens. Such methods may be the only ones of choice in certain situations. For the detection and quantitation of these antigens, it is very essential that the immunohistochemical methods for detecting them are properly standardized, with the inclusion of appropriate controls.