{"title":"共聚焦激光扫描显微镜对细胞凋亡的敏感性分析。","authors":"T Oite","doi":"10.1159/000020684","DOIUrl":null,"url":null,"abstract":"<p><p>Application of the TUNEL method and immunostaining cell-specific markers to a whole isolated glomerulus in combination with confocal laser scan microscopy can be used to analyze cell turnover including apoptosis within glomeruli. Furthermore, the technologies can be used to deepen our understanding of glomerular cell biology and pathophysiology at the cellular and molecular levels.</p>","PeriodicalId":12179,"journal":{"name":"Experimental nephrology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000020684","citationCount":"9","resultStr":"{\"title\":\"Sensitive analysis of apoptosis using confocal laser scan microscopy.\",\"authors\":\"T Oite\",\"doi\":\"10.1159/000020684\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Application of the TUNEL method and immunostaining cell-specific markers to a whole isolated glomerulus in combination with confocal laser scan microscopy can be used to analyze cell turnover including apoptosis within glomeruli. Furthermore, the technologies can be used to deepen our understanding of glomerular cell biology and pathophysiology at the cellular and molecular levels.</p>\",\"PeriodicalId\":12179,\"journal\":{\"name\":\"Experimental nephrology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000020684\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental nephrology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000020684\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental nephrology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000020684","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Sensitive analysis of apoptosis using confocal laser scan microscopy.
Application of the TUNEL method and immunostaining cell-specific markers to a whole isolated glomerulus in combination with confocal laser scan microscopy can be used to analyze cell turnover including apoptosis within glomeruli. Furthermore, the technologies can be used to deepen our understanding of glomerular cell biology and pathophysiology at the cellular and molecular levels.