大鼠乙酰辅酶a转运体cDNA克隆及其脑内表达模式。

R S Bora, S Ichikawa, A Kanamori, Y Hirabayashi
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引用次数: 7

摘要

大鼠乙酰辅酶a转运蛋白基因(Acatn)编码一种疏水多跨膜蛋白,参与神经节苷的o -乙酰化。已发现o -乙酰化神经节苷在神经系统的胚胎发育中起重要作用。我们用PCR克隆方法分离到了大鼠Acatn cDNA。大鼠Acatn氨基酸序列与人类和小鼠的同源性分别为92%和96%。该mRNA在大脑各发育阶段均有表达。Acatn在胚胎和出生后大鼠中的表达高于成年大鼠。用原位杂交法分析了成年大鼠脑Acatn mRNA的细胞定位。Acatn mRNA在成年大鼠脑小脑、海马、下丘脑、皮层、嗅球、背侧和腹侧前嗅核神经元细胞中表达。
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cDNA cloning of putative rat acetyl-CoA transporter and its expression pattern in brain.

Rat acetyl-CoA transporter gene (Acatn) encodes a hydrophobic multi-transmembrane protein involved in the O-acetylation of gangliosides. O-acetylated gangliosides have been found to play important roles in the embryonic development of the nervous system. We have isolated rat Acatn cDNA by PCR cloning. The amino acid sequence of rat Acatn exhibited 92% and 96% homology with human and mouse sequences, respectively. The mRNA was expressed in brain at all developmental stages. Acatn expression was higher in embryonic and postnatal rats than in adult rats. Cellular localization of Acatn mRNA in adult rat brain was also analyzed by in situ hybridization. Acatn mRNA expression was detected in the neuronal cells of cerebellum, hippocampus, hypothalamus, cortex, olfactory bulb, and dorsal and ventral anterior olfactory nucleus in adult rat brain.

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