生成的抗重组ATM的单克隆抗体支持激酶活性。

Hybridoma Pub Date : 2000-08-01 DOI:10.1089/027245700429864

K J Alligood, M Milla, N Rhodes, B Ellis, K E Kilpatrick, A Lee, T M Gilmer, T J Lansing

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引用次数: 9

摘要

我们报道了两种单克隆抗体ATM A16.35和ATM D16.11的快速生成，它们结合突变的共济失调毛细血管扩张症(ATM)的激酶结构域。这些抗体是利用RIMMS策略对大肠杆菌表达的重组蛋白产生的。我们通过Western blot分析发现，ATM A16.35与ATM结合，而ATM D16.11在免疫沉淀中与天然ATM形成免疫复合物，而不中和激酶活性。

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Monoclonal antibodies generated against recombinant ATM support kinase activity.

We report on the rapid generation of two monoclonal antibodies, ATM A16.35 and ATM D16.11, that bind to the kinase domain of mutated ataxia telangiectasia (ATM). These antibodies were generated against E. coli-expressed recombinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with native ATM in immunoprecipitations without neutralizing kinase activity.

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